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Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry.

Mark Jonathan Niedre

Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry.

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Published .
Written in English


About the Edition

Photodynamic therapy (PDT) is an emerging treatment for cancer and other conditions. PDT relies on the combined action of light, photosensitive drugs and molecular oxygen to generate reactive oxygen species, the most important of which is widely believed to be singlet oxygen (1O2 ). 1O2 rapidly oxidizes surrounding biomolecules in tissue, resulting in the photodynamic effect.1O2 is known to undergo luminescence at 1270 run, a fact that is routinely verified in simple solutions. In biological tissues, the probability of 1O2 luminescence falls to approximately 10-8 due to the dense intracellular environment and drastically reduced 1O2 lifetime. As such, measurement of 1O2 luminescence in cells in vitro or in tissues in vivo has never been reliably demonstrated in the past.The design and experimental validation of a spatially-resolved 1O2 luminescence detection system (i.e. a 1O2 imager) is also presented, as well as a technique for determining the ground-state molecular oxygen concentration based on 1O2 kinetics. Future work includes animal tumor dose-response studies, evaluation of novel photosensitizing compounds, and design of a clinical dosimetry system. (Abstract shortened by UMI.)In this work, a study is presented in which 1O2 luminescence was measured for the first time during PDT of leukemia cells in vitro and normal rat tissue in vivo using a novel, near-infrared sensitive photomultiplier tube. Measurement of the kinetics of the photosensitizer and of 1O2 in these environments was also possible. Studies that demonstrate that this luminescence correlates strongly with PDT treatment response in cell suspensions and in the skin of normal mice sensitized with Amino-leveulinic acid induced protoporphyrin IX (ALA-PpIX) are also presented. These studies confirm the likely role of 1O2 in porphyrin based PDT, and are encouraging for the prospect of development of a clinical 1O2 luminescence PDT dosimetry system.

The Physical Object
Pagination179 leaves.
Number of Pages179
ID Numbers
Open LibraryOL22624918M
ISBN 100612944174

The Influence of Oxygen Depletion and Photosensitizer Tripletstate Dynamics During Photodynamic Therapy on Accurate Singlet Oxygen Luminescence Monitoring and Analysis of Treatment Dose Response. MT Jarvi, MJ Niedre, MS Patterson, BC Wilson Photochemistry and photobiology 87 (1), , Photodynamic therapy (PDT) employs a photoactive molecule named a photosensitizer (PS), light absorbed by the PS and the molecular oxygen present in tissues to generate reactive oxygen species (ROS) [].ROS, such as singlet oxygen, superoxide ion, or hydroxyl radical, lead to the oxidation of biologically relevant molecules and cause irreversible destruction of target .   Photodynamic therapy (PDT) uses photosensitizers and visible light in combination with molecular oxygen to produce reactive oxygen species (ROS) that kill malignant cells by apoptosis and/or necrosis, shut down the tumor microvasculature and stimulate the host immune system. The excited singlet state of oxygen (1O2) is recognized to be the main .


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Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry. by Mark Jonathan Niedre Download PDF EPUB FB2

Key words: photodynamic therapy, singlet oxygen, luminescence, enhancement, dosimetry *Corresponding author: e-mail: [email protected], Phone: +86Fax: +86 Photodynamic therapy (PDT) uses photosensitizers and visible light in combination with molecular oxygen to pro-duce reactive oxygen species Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry.

book that kill malignantCited by: As photodynamic therapy (PDT) continues to develop and find new clinical indications, robust individualized dosimetry is warranted to achieve effective treatments. We posit that the most direct PDT dosimetry is achieved by monitoring singlet oxygen (1O2), the major cytotoxic species generated photochemically during by:   Photodynamic therapy (PDT) is generally based on the generation of highly reactive singlet oxygen (1 O 2) through interactions of photosensitizer, light, and oxygen (3 O 2).These three components are highly interdependent and dynamic, resulting in variable temporal and spatial 1 O 2 dose deposition.

Robust dosimetry that accounts for this complexity could Cited by: The effective dosimetry for photodynamic therapy (PDT) can be specified by direct measurement of singlet oxygen ((1)O2) production.

The purpose of this study was to investigate the feasibility of. Introduction. Photodynamic therapy (PDT) is a clinically‐approved, minimally‐invasive therapeutic procedure that utilizes photosensitizer (PS) to generate cytotoxic reactive oxygen species (ROS) for the treatment of oncologic and non‐oncological conditions For PDT applications, the robust and comprehensive individualized dosimetry is essential for achieving.

A Comparison of Singlet Oxygen Explicit Dosimetry (SOED) and Singlet Oxygen Luminescence Dosimetry (SOLD) for Photofrin-Mediated Photodynamic Therapy Michele M. Kim 1,2, Rozhin Penjweini 1, Nathan R.

Gemmell 3, Israel Veilleux 4, Aongus McCarthy 5, Gerald S. Buller 5, Robert H. Hadfield 3, Brian C. Wilson 4 and Timothy C. Zhu 1,*. Photodynamic therapy explicit dosimetry is recently developed to better correlate with clinical outcome of photodynamic therapy (PDT), which is a.

Introduction. Photodynamic therapy (PDT) has been used as a noninvasive procedure for treating a variety of different tumors, including skin, esophageal, bladder, lung, and cervical cancers, 1 and many nontumoral disease conditions such as age-related macular degeneration.

2 Singlet oxygen (SO) is believed to be the major cytotoxic agent involved in Cited by: 8. Photodynamic therapy (PDT) is generally based on the generation of highly reactive singlet oxygen ((1)O(2)) through interactions of photosensitizer, light, and oxygen ((3)O(2)).

Although singlet oxygen (1O2) has long been proposed as the primary reactive oxygen species in photodynamic therapy (PDT), it has only recently been possible to detect it in biological systems by Cited by:   Photodynamic therapy (PDT) is an important treatment modality for cancer and other localized diseases.

In addition to PDT dose, singlet oxygen (1 O 2) Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry. book is used as Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry.

book explicit Development and validation of singlet oxygen luminescence-based photodynamic therapy dosimetry. book dosimetry quantity, because 1 O 2 is the major cytotoxic agent in photodynamic therapy, and the reaction between 1 O 2 and tumor tissues/cells determines Cited by: 6.

Singlet oxygen (1 O 2) is believed to be the major cytotoxic agent involved in photodynamic therapy (PDT).Measurement of 1 O 2 near‐infrared (NIR) luminescence at nm in biological environments is confounded by the strongly reduced 1 O 2 lifetime and probably has never been achieved.

We present evidence that this is now possible, using a new. Accurate photodynamic therapy (PDT) dosimetry is critical for the use of PDT in the treatment of malignant and nonmalignant localized diseases. A singlet oxygen explicit dosimetry (SOED) model has been developed for in vivo purposes.

It involves the measurement of the key components in PDT—light fluence (rate), photosensitizer concentration, and ground-state oxygen Cited by: Singlet oxygen (1O2) is widely considered to play a major role in photodynamic therapy (PDT), and thus an increasing attention has been focused on the direct detection of 1O2 near-infrared.

Photodynamic therapy (PDT) is a therapeutic method for cancer and non-malignant disease treatment. Epigrammatically, it is based on the concept of the selective absorption of monochromatic light by appropriate chemical compounds - photosensitizers (PS) that are selectively localized in neoplastic tissue, in the presence of by: 8.

A Comparison of Singlet Oxygen Explicit Dosimetry (SOED) and Singlet Oxygen Luminescence Dosimetry (SOLD) for Photofrin-Mediated Photodynamic Therapy Michele M.

Kim, 1, 2 Rozhin Penjweini, 1 Nathan R. Gemmell, 3 Israel Veilleux, 4 Aongus McCarthy, 5 Gerald S. Buller, 5 Robert H. Hadfield, 3 Brian C.

Wilson, 4 and Timothy C. Zhu 1, *Cited by: Photodynamic Therapy Treatment. An optical fiber with a microlens attachment was coupled with a nm diode laser with a maximum output power of 8 W (B&W Tek Inc., Newark, Delaware) to produce a collimated beam with a diameter of 1 cm on the surface of the tumor [].Mice were treated with in-air fluence rates (φ air) of 50 to mW / cm 2 and total in-air Cited by: T1 - A comparison of singlet oxygen explicit dosimetry (SOED) and singlet oxygen luminescence dosimetry (SOLD) for photofrin-mediated photodynamic therapy.

AU - Kim, Michele M. AU - Penjweini, Rozhin. AU - Gemmell, Nathan R. AU - Veilleux, Israel. AU - McCarthy, Aongus. AU - Buller, Gerald Stuart. AU - Hadfield, Robert H. AU - Wilson, Brian by:   Singlet oxygen (1 O 2) is the major cytotoxic agent responsible for cell killing for type-II photodynamic therapy (PDT).An empirical four-parameter macroscopic model is proposed to calculate the “apparent reacted 1 O 2 concentration”, [1 O 2] rx, as a clinical PDT dosimetry model incorporates light diffusion equation and a set of PDT kinetics.

Singlet oxygen (1O2) is widely considered to play a major role in photodynamic therapy (PDT), and thus an increasing attention has been focused on the direct detection of 1O2 near-infrared luminescence around nm for PDT dosimetry. A new sensitive detection system is developed to directly measure the temporal and spectral resolved 1O2 luminescence spectra.

Thesis: “Development and Validation of Singlet Oxygen Luminescence-Based Photodynamic Therapy Dosimetry” McMaster University, Hamilton, Ontario, Canada in Engineering Physics, April 2. ACADEMIC APPOINTMENTS 08/ – present Associate Professor, Northeastern University, Department of Electrical and Computer Engineering, Boston, MA.

Although singlet oxygen (1 O 2) has long been proposed as the primary reactive oxygen species in photodynamic therapy (PDT), it has only recently been possible to detect it in biological systems by its luminescence at previously demonstrated this in vitro and in vivo, we showed that cell survival was strongly correlated to the 1 O 2 luminescence in.

photochemical parameters in vivo for singlet oxygen based dosimetry of PDT. Keywords: Singlet oxygen, BPD-PDT, finite element method, necrosis.

Introduction Photodynamic therapy (PDT) is an emerging treatment modality for malignant and non-malignant conditions. PDT combines a light. A singlet oxygen explicit dosimetry (SOED) model has been developed for in vivo purposes.

It involves the measurement of the key components in PDT-light fluence (rate), photosensitizer concentration, and ground-state oxygen concentration ([³O₂])-to calculate the amount of reacted singlet oxygen ([¹O₂]rx), the main cytotoxic component in Cited by: Introduction.

Photodynamic therapy (PDT) is a dynamic interaction between the photosensitizer, oxygen, and light 1, use for treatment of age‐related macular degenerationactinic keratosis 6, 7, esophageal cancers 8, 9, and non‐small cell lung can 11 has been approved by the FDA.

The use of PDT in pre‐clinical studies and clinical trials for skinbladder 15 Author: Michele M. Kim, Arash Darafsheh. Singlet oxygen (1O2), molecular oxygen in the lowest excited state, has a critical role in the cell-killing mechanism of photodynamic therapy (PDT).

Although 1O2 phosphorescence measurement has been mainly used to monitor 1O2 formation during PDT, its intensity is far insufficient to obtain two-dimensional images of intracellular 1O2 with the subcellular spatial Cited by: Far-Red Fluorescence Probe for Monitoring Singlet Oxygen during Photodynamic Therapy Sooyeon Kim, Takashi Tachikawa, Mamoru Fujitsuka, and Tetsuro Majima* Contents (1) Experiment methods (2) Synthesis and characterization of Si-DMA (3) Supporting figures (Figure S1–S14) (4) References (1) Experimental methods Characterizations of Si-DMA.

Singlet oxygen is a primary cytotoxic agent in photodynamic therapy. We show that CeF3 nanoparticles, pure as well as conjugated through electrostatic interaction with the photosensitizer Cited by:   The research in the field of the photodynamic therapy of cancer (PDT) is focused on a development of photosensitizers exhibiting high quantum yield of singlet oxygen production.

Direct time-resolved spectroscopic observation of singlet oxygen phosphorescence can provide time constants of its population and depopulation as well as photosensitizer Cited by: Singlet oxygen (1 O 2) is thought to be the cytotoxic agent in photodynamic therapy (PDT) with current monitoring of 1 O 2 concentration in vivo would be a valuable tool in studying biological response.

Attempts were made to measure 1 O 2 IR luminescence during PDT of cell suspensions and two murine tumour models using the photosensitizers Photofrin II Cited by: The lack of accurate and robust photodynamic therapy dosimetry is one of the obstacles for the widespread clinical applications.

In this study, we propose a methodology to monitor the production of reactive oxygen species in real-time using the phosphorescent spectra of metalloporphyrin based photosensitizer.

The correlation among the phosphorescence. Singlet oxygen luminescence as an in vivo photodynamic therapy dose metric: validation in normal mouse skin with topical amino-levulinic acid.

Br J Cancer. ;– Br J Cancer. ;–Cited by: 1. Direct monitoring of singlet oxygen (1O2) luminescence is a particularly challenging infrared photodetection problem.

1O2, an excited state of the oxygen molecule, is a crucial intermediate in many biological processes. We employ a low noise superconducting nanowire single-photon detector to record 1O2 luminescence at nm wavelength from a model photosensitizer.

Singlet oxygen (1 O 2) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT).In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated 1 O 2 luminescence.

The validation of Cited by: 9. The efficiency of photodynamic action caused by single short laser pulse can be evaluated as: (4) E = Q ∫ Δ t n Δ t dt, where E is the mass of oxidized contents, Q the dimensional coefficient, n Δ (t) -the singlet oxygen concentration and Δt is the time of singlet oxygen presence in the cell.

It is believed that the concentration of Cited by: The energy level diagram for the excitation of singlet oxygen by energy transfer from the triplet T 1 state of the photosensitizer and the deactivation of singlet oxygen and the T 1 state is shown schematically in Fig.

contains the singlet ground state S 0 of the photosensitizer, its first excited singlet state S 1 and its first excited triplet state T by: Photodynamic therapy (PDT) involves the administration of a photosensitizer (PS) that after exposure to light, and in the presence of oxygen, leads to the formation of reactive oxygen species, predominantly singlet oxygen [].The efficacy of the treatment is dependent on many factors that dynamically interact during : Wei Peng, Henriette S.

de Bruijn, Timo L. ten Hagen, Kristian Berg, Jan L. Roodenburg, Go M. Singlet oxygen (1O2) is the major cytotoxic agent responsible for cell killing for type‐II photodynamic therapy (PDT). An empirical four‐parameter macroscopic model is proposed to calculate the “apparent reacted 1O2 concentration”, (1O2)rx, as a clinical PDT dosimetry quantity.

This model incorporates light diffusion equation and a set of PDT kinetics. Singlet oxygen is a highly reactive species which is involved in a number of processes, including photodynamic therapy of cancer.

Its very weak near-infrared emission makes imaging of singlet oxygen in biological systems a long-term challenge. We address this challenge by introducing Singlet Oxygen Feedback.

The aim of this study was to perform a systematic review of the literature followed by a meta-analysis about the efficacy of photodynamic therapy (PDT) on the microorganisms responsible for dental caries.

The research question and the keywords were constructed according to the PICO strategy. The article search was done in Embase, Lilacs, Scielo, Medline, Scopus, Cited by: 1. 1. Introduction. Photodynamic therapy (PDT) is a superordinate concept for treatments pdf on the photosensitized generation of singlet oxygen.

1, 2 Singlet oxygen (O 2 1) is molecular oxygen in its lowest excited electronic is highly reactive and thus one of the reactive oxygen by: Photodynamic therapy (PDT) treatments were performed on suspensions of AML5 download pdf incubated with ALA-induced PpIX with either 15 mW/cm 2, 45 mW/cm 2 or 90 mW/cm 2 pulsed nm light.

Singlet oxygen (1 O 2) luminescence measurements were carried out using a detection system including a near-infrared (NIR) sensitive photomultiplier tube (PMT).Cell Cited by: 6.The amidation reaction between C 60 with an activated ester group (1) and ebook Glc ebook with an amino group (2) was performed to yield the target AcGlc-pendant C 60 compound (3).

The water soluble deacetylated compound, Glc-pendant C 60 compound (4), exhibited high photocytotoxicity against HeLa cells due to the more efficient singlet oxygen generation as Cited by: 7.